Production of marmoset eggs and embryos from xenotransplanted ovary tissues

Sci Rep. 2023 Oct 24;13(1):18196. doi: 10.1038/s41598-023-45224-x.

Hirayama R1,3,7, Taketsuru H2,7, Nakatsukasa E1, Natsume R1, Saito N2, Adachi S2, Kuwabara S2, Miyamoto J2, Miura S2,4, Fujisawa N2, Maeda Y2, Takao K3,5,6, Abe M1, Sasaoka T2, Sakimura K1.

1Department of Animal Model Development, Brain Research Institute, Niigata University

2Department of Comparative and Experimental Medicine, Brain Research Institute, Niigata University

3Department of Behavioral Physiology, Graduate School of Innovative Life Science, University of Toyama

4Institute for Research Administration, Niigata University

5Department of Behavioral Physiology, Faculty of Medicine, University of Toyama

6Research Center for Idling Brain Science, University of Toyama

7These authors contributed equally: Runa Hirayama and Hiroaki Taketsuru

The common marmoset (Callithrix jacchus) has attracted attention as a valuable primate model for the analysis of human diseases. Despite the potential for primate genetic modification, however, its widespread lab usage has been limited due to the requirement for a large number of eggs. To make up for traditional oocyte retrieval methods such as hormone administration and surgical techniques, we carried out an alternative approach by utilizing ovarian tissue from deceased marmosets that had been disposed of. This ovarian tissue contains oocytes and can be used as a valuable source of follicles and oocytes. In this approach, the ovarian tissue sections were transplanted under the renal capsules of immunodeficient mice first. Subsequent steps consist of development of follicles by hormone administrations, induction of oocyte maturation and fertilization, and culture of the embryo. This method was first established with rat ovaries, then applied to marmoset ovaries, ultimately resulting in the successful acquisition of the late-stage marmoset embryos. This approach has the potential to contribute to advancements in genetic modification research and disease modeling through the use of primate models, promoting biotechnology with non-human primates and the 3Rs principle in animal experimentation.


*Reprinted under the terms of the Creative Commons Attribution License (CC BY).


Related BRI Department

Research Findings